Airway neutrophils and myeloperoxidase in asthma patients with cold-induced bronchoconstriction

Introduction. The role of neutrophil inflammation the bronchi in patients with asthma devel­opment and manifestation cold airway hyperresponsiveness (CAHR) has not been sufficiently studied. Aim. To assess level neutrophils activity myeloperoxidase (MPO) cold-induced bronchospasm. Materials methods. In 138 mild-to-moderate CAHR, control (ACT), lung function (FEV 1 ), cellular composition sputum was examined before after bronchoprovo­cation test by isocapnic hyperventilation air (IHCA). samples, percentage neutrophils, eosino­phils, bronchial epithelial cells (structurally intact cylindrical ciliated goblet cells) assessed; concentration MPO were studied cytochemical method. Results. divided into three groups: group (n=94) - mild bronchospasm, 2 (n=28) moderate broncho­spasm, 3 (n=16) severe bronchospasm (AFEV =- 13.5±0.3, -24.6±0.5, -36.9±1.5%, respectively). Patients did differ (16 [12; 21]; 16 [13; 20] 21] ACT points, third had lower values patency comparison first second FEV 79.4±3.2; 92.4±1.7 (p<0.01); 92.1±2.9% FEF 25-75 46.4±4.3; 66.1±2.5 (p<0.001); 63.2±4.0% (p<0.01). IHCA groups 3, an increase number recorded from 35.5±3.9 to 46.0±3.8% (p<0.05) 39.0±3.8 52, 4±4.4% (p<0.05), respectively, while change (43.2±2.4 44.3±2.1%). response all significantly increased more than 30% (p<0.05). Conclusion. mobilization a mixed pattern pa­tients is associated worsening An content gran­ules action aimed at enhancing secretion enzyme interstitium, activating its oxidative respiratory tract, escalating oxidative/halogenating stress that accompanies broncho­spasm.